Numbers 1:32-33

32 ¶ Of the children of Joseph, namely, of the children of Ephraim, by their generations, after their families, by the house of their fathers, according to the number of the names, from twenty years old and upward, all that were able to go forth to war; 33 Those that were numbered of them, even of the tribe of Ephraim, were forty thousand and five hundred.